Measurement

Part:BBa_J100273:Design

Designed by: Anna Buser   Group: Campbell M Lab   (2016-06-24)


tCloneTet+PDB riboswitch with P2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 361
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 507
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
    Illegal NgoMIV site found at 901
    Illegal NgoMIV site found at 1061
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used PCR to "cut out" the P5 promoter since there were no restriction enzyme sites surrounding it.


Source

J100255

References